Off-the-Shelf Plasmids for AAV and LV
Improve Your Viral Vector Process
Complementary to our transfection reagents and cell culture media for gene therapy, we have developed pPLUS® off-the-shelf packaging plasmids for AAV and LV production. These innovative plasmids enhance efficiency and productivity, providing a reliable solution for researchers and manufacturers. With optimized features, ready-to-use formats, and availability in Research, High Quality, and GMP grades, pPLUS® plasmids ensure high-quality results in gene therapy applications.
Explore Our Off-the-Shelf Packaging Plasmids
Effective gene therapy using AAV or LV vectors depends on careful plasmid design to improve vector yield, while ensuring regulatory compliance. Discover pPLUS® highly purified and optimized plasmids engineered to make your gene therapy a success.
Complete Plasmid Porfolio Enhancing AAV Production
pPLUS® AAV is a set of three plasmids pPLUS® AAV-Helper, pPLUS® AAV-RepCap and pPLUS-AAV eGFP transgene designed for AAV production of various serotypes. These plasmids are optimized to work together, with FectoVIR®-AAV in a triple transfection process, providing enhanced AAV titers and infectivity. They are readily available from Research grade to GMP
Improved performance used independently or in combination: Higher VG titers , Higher F/E ratios
Readily Available: Available in different pack sizes and in different quality grade R&D, HQ and GMP grade
Scalable: Fine-tuned design to derisk large scale production
Cost-Saving: License and royalty-free products
pPLUS® AAV plasmids are optimized to produce higher titers with improved F/E ratio. compare to other commercially available 3-plasmid sets for AAV production. The use of pPLUS® AAV-Helper, pPLUS® AAV-RC2 and pPLUS® AAV-eGFP led to a 3-fold increase in VG and 2-fold increase in F/E ratio compared to competitor plasmid sets 1 and 2, following standard conditions.
Refined Plasmids for Lentivirus Production
Elevate your lentivirus production with pPLUS® LV, a designed set of GAG/POL, REV, and VSV-G plasmids. Engineered to seamlessly integrate with FectoVIR®-LV in a third-generation transfection process, these plasmids deliver enhanced yields with high-quality titers. They are readily available in Research grade and can be custom made to HQ and GMP grade.
- Efficient: Best industry performance
- Readily Available: Available on-demand in different pack sizes, for an easy and fast supply
- Scalable: Available in different quality grades from Research to GMP, supporting seamless scale-up
- Compatible: With various production systems (transfection reagents and cell lines)
- Cost-Saving: Royalties and licenses free
FAQs - pPLUS® Plasmids
There is no minimum ordering quantity.
Sartorius recommends adjusting the plasmid ratios according to your production process. To determine the optimum transfection conditions, including pDNA ratios for rAAV or LV production, Sartorius recommends using a Design of Experiment (DoE). For plasmid ratios optimization, Sartorius recommends using a Mixture Design approach which is the most appropriate for this purpose.
Sartorius can provide support in building a DoE experimental plan and analyzing the results. If you would like a more complete DoE proposition adapted to your goals and constraints, feel free to contact support here.
pPLUS® are shipped in dry ice. Fee for the dry ice packaging will be charged to customers and included in the shipment cost. This will be automatically indicated in the quote, in the product description table.
Our plasmids are license and royalty-free, even though pPLUS® AAV-Helper is patented. Sartorius remains the sole provider, meaning that amplification of the plasmid cannot be performed with any manufacturer other than Sartorius. For more information, please refer to the terms and conditions of our products.
